Yu etal_ISCIENCE-D-23-06561: A Non-Viral DNA Delivery System Consisting of Multifunctional Chimaeric Peptide Fused with Zinc Finger Protein

Non-viral gene delivery systems have received sustained attention as a promising alternative to viral vectors for disease treatment and prevention in recent years. Numerous methods have been developed to enhance gene uptake and delivery in the cytoplasm, however, due to technical difficulties and delivery efficiency, these systems still face challenges in a range of biological applications, especially in vivo. To alleviate this challenge, we devised a novel system for gene delivery based on a recombinant protein eTAT-ZF9-NLS, which consisted of a multifunctional chimaeric peptide and a zinc finger protein with sequence-specific DNA-binding activity. High transfection efficiency was observed in several mammalian cells after intracellular delivery of plasmid containing ZF9-binding sites mediated by eTAT-ZF9-NLS. Our new approach provides a novel transfection strategy and the transfection efficiency was confirmed both in vitro and in vivo, making it a preferential transfection reagent for possible gene therapy.
The excel files are arranged figure wise and contain the raw data used to generate figures shown in the manuscript "ISCIENCE-D-23-06561".

近年来，非病毒基因递送系统因其作为疾病治疗与预防中病毒载体替代品的巨大潜力而持续受到广泛关注。众多方法被开发出来以增强细胞质中的基因摄取和递送，然而，由于技术难题和递送效率的限制，这些系统在多种生物学应用中，尤其是在体内应用中，仍面临挑战。为了缓解这一挑战，我们设计了一种基于重组蛋白eTAT-ZF9-NLS的新型基因递送系统，该系统由多功能嵌合肽和具有序列特异性DNA结合活性的锌指蛋白组成。在将包含ZF9结合位点的质粒通过eTAT-ZF9-NLS介导进行细胞内递送后，在多种哺乳动物细胞中观察到高转染效率。我们的新方法提供了一种新颖的转染策略，其转染效率在体外和体内均得到证实，使其成为可能基因治疗的优选转染试剂。Excel文件按照图示排列，包含用于生成论文“ISCIENCE-D-23-06561”中所示图象的原始数据。