LentiCRISPR-Cas9-2A-mCherryU6ZF vector
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下载链接:
https://data.mendeley.com/datasets/k25kdh7z25
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资源简介:
The plasmid (15, 126 pb) was constructed encoding the spCas9 protein driven by the EFS (mammalian) promoter in a bicistronic cassette that also expressed mCherry. For the expression of the sgRNA, a cassette containing the zebrafish U6 RNA III polymerase (U6ZF) promoter was used. The new plasmid was able to display the expression for Cas9, mCherry and sgRNA in CHSE/F fish cell.
创建时间:
2018-11-29



