Spatial Transcriptomics of Lungs Infected with Coccidioides posadasii

Coccidioidomycosis, or Valley Fever, is a lung disease caused by inhaling Coccidioides fungi, prevalent in the Southwestern U.S., Mexico, and parts of Central and South America. Climate change is contributing to the spread of this disease. Many cases are asymptomatic, some are often misdiagnosed, and a small subset can progress to severe illness. To better understand lung responses during late Coccidioides infection, we used 10x Visium spatial transcriptomics. We analyzed non-infected lung (D0) and infected lung at 14 days post-infection (D14). Overall design: Spatial transcriptomics was conducted on lung tissues from mice intranasally infected with Coccidioides posadasii. Samples were taken from a non-infected lung (D0) and an infected lung 14 days post-infection (D14).