Mapping ISG15 sites on GAPDH and PGK1 by AP-MS
收藏NIAID Data Ecosystem2026-05-10 收录
下载链接：
https://www.omicsdi.org/dataset/pride/PXD071724
下载链接
链接失效反馈官方服务：
资源简介：
We employed an AP-MS approach to map ISG15 modification sites on GAPDH and PGK1 in HEK293T cells by overexpression of FLAG-GAPDH, FLAG-PGK1 or FLAG-GFP together with the ISG15 conjugation enzymes (E1, E2, E3) and wild-type HA-ISG15 or conjugation-defective ISG15-AA. Cells were lysed in physiological buffer, subjected to FLAG-pulldown followed by on-bead digestion and further processing for LC-MS/MS analysis.
创建时间：
2026-03-02
