CRISPR and biochemical screens identify MAZ as a co-factor in CTCF-mediated insulation at Hox clusters [MAZ KO]

In this experiment, we sought to analyze how transcriptome of WT and MAZ knock-out (KO) cells change during differentiation of mouse embryonic stem cells (ESCs) into cervical motor neurons (MNs) Examination of gene expression of ESCs and cervical MNs using ribo-zero RNAseq, by knock-out status of MAZ in triplicates, 2 cell lines (WT and MAZ KO)