ChIP-Seq of Jmjd2a and Jmjd2c in B220+ B cells

Here we reported the Jmjd2a and Jmjd2c are co-targeted at the essential genes of mouse activating B220+ B cells. B cell activation programs transcription networks that subsequently induce plasma cell differentiation, but how histone demethylases participating in this process remains elusive. We found that histone demethylases Jmjd2a and Jmjd2c are expressed in Tfh-mediated signals stimulated mouse spelenic B220+ B cells. we then perform ChIP-seq by using anti-Jmjd2a and anti-Jmjd2c antibodies to identified the genes regulated by Jmjd2a and Jmjd2c and required for correct activation. Overall design: Examination the genes which are regulated by two different histone demeltylases, Jmjd2a and Jmjd2c in Tfh-mediated signals stimulated B220+ B cells by ChIP-seq.