Illumina miRNA sequencing revealed Citrus adaptation to long-term B-toxicity through modulation of lateral root development by miR319

We report the application of Illumina sequencing for high-throughput profiling of miRNA in citrus root responded to long-term boron toxicity. We find miR319 is involved in citrus adapation to long-term boron toxicity via targeting a MYB gene, Ciclev10000756m.g.v1.0, which is homologus with several MYBs that modulate lateral root development in Arabidopsis. Overall design: Citrus sinensis and C. grandis seedlings were sand cultured and treated with different levels of H3BO3 for 15 weeks. At the end of B treatments, root tips were used for total RNA extraction. small RNA libraries were then constructed and sequenced with an Illumina HiSeq 2500.