OMAP-12: Organ Mapping Antibody Panel (OMAP) for Multiplexed Antibody-Based Imaging of Human Retina with IBEX, v1.0

OMAP-12 was designed for IBEX imaging of fixed frozen human retina samples<br>(https://doi.org/10.1038/s41596-021-00644-9; https://doi.org/10.1073/pnas.2018488117). The panel contains 14<br>antibodies, 3 lectins, and DAPI for image alignment and nuclear segmentation. The OMAP provides a spatial context for<br>the main anatomical structures and key cell types present in the healthy human retina including photoreceptors,<br>bipolar cells, ganglion cells, astrocytes, microglia, Muller glia, and retinal vascular endothelium. Broad neuronal<br>classes are imaged, but not separated into fine subpopulations partly due to the lack of antibody reagents (e.g., all<br>amacrine, ganglion cell, and bipolar cell subtypes). Fluorescent lectins PNA, Isolectin B4, and Phalloidin were used<br>to visualize oligosaccharides for typing cell types such as cone photoreceptors, vascular endothelium, and outer<br>limiting membrane, respectively. These reagents are visualized in the accompanying dataset but not included in the<br>OMAP because they are not antibodies. Additional details on sample preparation and imaging can be found here: (doi:<br>10.1038/s41596-021-00644-9) Custom conjugations were performed for several antibodies according to the standard<br>manufacturer protocols. A representative dataset acquired using this OMAP has been uploaded here:<br>https://doi.org/10.5281/zenodo.7777285.