Multi-omics analysis of a dual substrate, N2O respiring enrichment culture

The manipulation of soil microbiomes and their metabolisms requires massive inoculation with specialist microbes capable of growth and activity in both the inoculation vector as well as soil. A recent enrichment approach utilising dual substrate sequential enrichments in digestate and soil has yielded isolates capable of growth and activity in both materials. Here we use metagenomics and metaproteomics to investigate the enrichment cultures and isolated organisms in order to understand the genetic basis and expressed proteins responsible for success under these growth regimes which have resulted in organisms suited to growth and activity in both materials.