Hematological profile data of individuals exposed to mercury in the interior of the Amazon

Human contamination of Hg occurs through the consumption of fish contaminated with MeHg, an organic form and the most toxic of the metal, due to its ability to bioaccumulate and biomagnify in the trophic chain. Considering that studies carried out in riverside communities in the Amazon have already revealed high levels of Hg in fish from this region and high levels of total Hg and MeHg in these populations, configuring a chronic exposure in these communities, the data presented refer to the hematological profile of individuals from different locations. from the municipality of Santarém, Pará (urban area, plateau and floodplain). These data are linked to the Research Protocol approved by the CEP of the State University of Pará-UEPA, under the opinion of 1,127,108. To obtain the data, a semi-structured questionnaire was applied and blood was collected. After answering the questionnaire and signing the informed consent, 10 mL of sample was collected by venipuncture from each volunteer. This amount was divided into two 5 mL tubes with an EDTA additive. One tube was intended for carrying out the blood count and the other was intended for the measurement of Hg. The tube for performing the blood count was kept at room temperature until the time of execution, and the period between collection and analysis did not exceed 24 hours. The tube intended for the measurement of Hg was frozen. And Hg dosages were performed within a period of up to 15 days after collection. The Hg was measured in the laboratory of Bioprospection and Molecular Biology at Ufopa. The equipment used was the DMA-80 Direct Mercury Analyzer (Milestone), which expresses the Hg values through the atomic absorption spectrophotometry technique. All samples were performed in duplicates to increase the reliability of the expressed value. The reference value used for Hg was established by the WHO (1990), with a value of up to 10 μg/L being considered acceptable. For the exposure classification, all individuals who presented values above 10 μg/L were considered exposed. The blood count was performed on the MINDRAY BC 5380 equipment, fully automated and interfaced. The procedure was divided into two parts: automated reading and microscopic analysis. For microscopic analysis, a blood smear was made and subjected to rapid panopticon staining. Then the slides were analyzed microscopically to describe the cell morphology and to verify if the microscopic part was consistent with the automation. A double-blind control was performed for each batch of ten slides by two pharmaceutical microscopists specialized in clinical analysis. These data arouse the interest of better understanding the clinic of individuals environmentally exposed to Hg in the interior of the Amazon, since the signs and symptoms of exposure are very unspecific, and can, therefore, be confused with the signs and symptoms of diseases with the diagnostic plan. already defined.

汞（Hg）的人体污染主要通过摄入被甲基汞（MeHg）污染的鱼类而发生，甲基汞作为一种有机汞，其毒性最高，这主要归因于其在食物链中的生物累积和生物放大能力。鉴于在亚马逊河流域社区进行的研究已揭示该地区鱼类中汞含量较高，以及这些人群中总汞和甲基汞含量较高，形成了对这些社区的慢性暴露，所提供的数据涉及不同地区个体的血液学特征。这些数据来源于帕拉州桑塔雷姆市（城市区域、高原和冲积平原）。这些数据与帕拉州立大学-UEPA的CEP批准的研究方案相联系，方案编号为1,127,108。为了获取数据，应用了半结构化问卷，并采集了血液样本。在回答问卷并签署知情同意书后，从每位志愿者体内采集了10毫升样本，该样本分为两个5毫升的试管，其中一个添加了EDTA添加剂。一个试管用于进行血细胞计数，另一个用于汞的测量。用于血细胞计数的试管在室温下保存，直至执行，采集与分析之间的时间不超过24小时。用于汞测量的试管则进行冷冻处理。汞的测量在乌法帕生物勘探和分子生物学实验室进行，使用的设备是DMA-80直接汞分析仪（Milestone），该设备通过原子吸收分光光度法技术表达汞值。所有样本均进行双份检测，以提高表达值的可靠性。汞的参考值由世界卫生组织（WHO，1990年）设定，认为10 μg/L以下的汞值是可接受的。对于暴露分类，所有汞值超过10 μg/L的个体被视为暴露。血细胞计数在MINDRAY BC 5380设备上完成，该设备为全自动且具有接口。程序分为两部分：自动化读取和显微镜分析。对于显微镜分析，制作了血涂片并进行了快速全视野染色。然后，通过显微镜分析这些切片，以描述细胞形态并验证显微镜部分是否与自动化结果一致。每批十张切片由两位专门从事临床分析的药品显微镜学家进行双盲控制。这些数据引起了人们对于理解亚马逊内地环境暴露于汞的个体临床特征的兴趣，因为暴露的迹象和症状非常不特异，因此可能与其他已定义的诊断计划的疾病症状相混淆。