Identification of potential in vivo targets for rMCP-2.

By screening of the entire rat proteome with a 4 amino acid peptide sequence having the following sequence: P2 (L/V), P1 (F/Y), P1' (S/A/V/R), P2' (A/S/V) resulted in 4825 rat specific hits. This sequence was derived from the rMCP-2 cleavage consensus sequence. Nuclear and cytoplasmic proteins were removed from the list, as they most likely do not represent likely in vivo targets. Among these originally 4825 hits, 78 were considered to be particularly interesting and could be considered as potential substrates for rMCP2 and these are listed in this table.Identification of potential in vivo targets for rMCP-2.