Expression data from HEK293T cells

cGAS-STING expression in HEK293T cells could up regulate a series of genes. Above them, Vpx significantly suppressed the NF-κB activated genes but had only a slight influence on the IRF3-activated genes. Diverse HIV-2 and SIV Vpx proteins showed an ability to inhibit DNA sensing-activated innate immune responses, suggesting an evolutionarily conserved function for Vpx. The cGAS, STING, and HIV-2/SIV Vpx or its control vector were transfected in HEK293T cells. Twenty hours later, mRNA samples were prepared and analyzed using Affymetrix microarrays. We sought to obtain up-regulated genes by cGAS-STING and to which of them were suppressed by Vpx specifically.