RNA-seq of K1 papillary thyroid cancer (PTC) cell with different TSC22D1 overexpression status

RNA sequencing was performed on K1 papillary thyroid cancer (PTC) cells to investigate the biological function of TSC22D1 variant 2 overexpression. Cells were transfected with the TSC22D1 (NM_006022) Human Tagged ORF Clone, with three empty vector-transfected cells ((TSC22D1_0) serving as negative controls. Experimental groups included four replicates each of cells with low (TSC22D1_200) and high levels (TSC22D1_500) of TSC22D1 variant 2 overexpression. RNA integrity was confirmed for all samples using the Agilent TapeStation system, with RNA integrity numbers (RIN) exceeding 7.0.