Time-resolved X-ray solution studies of pH induced conformational changes in a proton-sensitive potassium channel

ESRF Portal2028-01-01 更新2026-04-23 收录

离子通道动力学

pH敏感性

数据链接：

https://doi.esrf.fr/10.15151/ESRF-ES-2005461840 数据链接链接失效反馈

官方服务：

资源简介：

We will use time-resolved X-ray solution scattering (TR-XSS) to study the pH sensitive ion channel KcsA. An earlier experiment at ID09 (LS-3224) was very successful, with changes in the X-ray scattering observe when UV light was used to release caged protons, thereby jumping the sample from pH 7 (neutral) to pH 4 (acidic). We will build from this earlier experiment to test if point-mutations that inactivate the enzyme also suppress the observed conformational changes. Since the release of photocaged protons is very fast, we will also collect data for early-microsecond delays, aiming to measure the rise of the pH induced conformational change in KcsA. Finally, we will reproduce the pH induced conformational change in KcsA using a mixing flow-cell, with the goal of developing a generic approach to TR-XSS. Our studies of KcsA mutants will provide new structural insight into the transition from a closed to an open conformation of this highly studied potassium channel.

提供机构：

The Faculty of Science, Dept. of Chemistry and Molecular Biology, Medicinaregatan 9C, 405 30, Goeteborg, SE; Goeteborg University , Department of Biochemistry & Biophysics, PO Box 462, 40 530 Goeteborg, Sweden; Goeteborg University , Department of Biochemistry & Biophysics, PO Box 462, 40 530 , Goeteborg, SE; Lundberg Laboratory, Dept of Chemistry and Molecular Biology, PO Box 462, 405 30 Goeteborg, Sweden; Goeteborg University, Lundberg Laboratory, Dept of Chemistry - Biochemistry, Medicinaregatan 9e, 41 390 Goeteborg, Sweden; The Faculty of Science, Dept. of Chemistry and Molecular Biology, Medicinaregatan 9C, 405 30 Goeteborg, Sweden

创建时间：

2028-01-01