The role of the cAMP/PKA pathway and Com2 in regulating the activity of UASru.

Com2Δ cells did not grow on acetate medium. Therefore, com2 mutants and their isogenic wt strain were grown in SD medium to either logarithmic stage (SD) or stationary stage (SA). The results are expressed as relative β-galactosidase activity and represent the average data for three independent transformants. Strains used were as follows: wt (Y1265) and its isogenic strain gpr1Δ/gpr1Δ (Y1267), ras2Δ/ras2Δ (Y1270), cdc25Δ/cdc25Δ carrying CDC25ΔSH3 on a URA3 CEN vector (Y1338-2), a wt haploid (Y1890) and its isogenic strains com2Δ (Y1968) and com2S164AS88A (Y2052), a wt haploid (Y1214) strain and its isogenic strain sko1Δ (Y2063). These strains carry the lacZ reporter genes integrated within genomic LEU2. NT – Not Tested.