Dissociation temperatures and kinetics of wild-type YscUC and the suppressor mutants V292T, Y287G, and A268F probed with NMR and CD spectroscopy.

CD spectroscopy at 220 nm was performed at a scan rate of 1°C/min to determine the dissociation temperature (Tdiss) of YscUC and suppressor mutants (Figure 7A). The kinetics of the dissociation process (τdiss) was monitored with CD spectroscopy at 220 nm and NMR spectroscopy at 37°C and 30°C. See also Figure 7B and 7C; Figure S5B.ameasured by following the CD signal at 220 nm;bmeasured with a scan-rate of 1°C/min;cdissociation was too slow to fit with a single exponential decay function;dmeasured by following methyl group intensities in one dimensional 1H spectra.