Transcriptional profiling of sorted tumor cells from implanted T cell low and T cell high mouse pancreatic tumors

Purpose: Use RNA-seq strategy to characterize the differentially expressed genes in T cell low and high mouse pancreatic tumors in order to identify factors contributing to the differential abundance of tumor infiltrating T cells. Methods: Subcutaneously implanted T cell low and high pancreatic tumors were utilized for the preparation of RNA-seq libraries from sorted tumor cells. Examination of differentially expressed genes between two biological groups (T cell low v.s. T cell high tumors).