Quantitative Analysis of different NK cell types Transcriptomes

We compared transcriptome of different types of NK cells including obtained from Umbilical Blood (UCB-NK) from 3 donors, both UCB56 NK cells that were isolated from the UCB unit as well as UCB34 NK cells that were differentiated from CD34+ cells, and derived from human iPSC (iPSC-NK), pre and post antigen presenting cell expansion. When comparing the similarity of the NK cells populations by the number of differentially expressed genes to iPSC NK cells, there were the pre and post expansion cells clustered together mRNA profiles of 4 different NK cell types from 3 donors and iPS NK cells were generated by deep sequencing, using Illumina Hi-seq 2000.