longfin causes cis-ectopic expression of the kcnh2a ether-a-go-go K+ channel to autonomously prolong fin outgrowth

longfin mutant zebrafish grow and regenerate abnormally long fins. We sought to determine misregulated transcripts in the distal regenerate of 96 hpa caudal fins that could contribute to fin overgrowth or be the longfin causal gene. We used RNA-Seq to compare gene expression between replicates of microdissected distal regenerating fin tissue, distinguished by GFP-negative tissue distal to sp7:GFP expressing cells, collected from longfin heterozygous adult fish and homozygous wildtype clutchmates. Each replicate consisted of pooled tissue from four caudal fins.