Tracheal aspirate bulk RNA sequencing from patients with ARDS [bulk RNA-seq]

Trachel aspirates samples were collected from an observational cohort of mechanically ventilated patients to compare respiratory tract gene expression in previous-descried ARDS molecular phenotypes. Patient charts were reviewed by two physicians and patients with ARDS were identified using the Berlin Definition. Control subjects were mechanically ventilated for neurologic indications, were not immunosuppressed, and had no evidence of pulmonary disease. ARDS molecular phenotypes were defined using a previously-described 3-variable classifier (Sinha 2020) Tracheal aspirates were collected from mechnically ventilated patients admitted to the intensive care units. ***Please note that the UCSF IRB has restricted sharing the raw data because some of the samples were collected under a waiver of consent and could in theory be identifiable. Therefore, raw data is not provided***