Dead-end inhibition patterns and inhibition constants for oxalate and ADP-Cr2+ in VcIPK and VcIIPK.

Inhibition patterns were taken from the double reciprocal plots of the inhibition experiments. Simple inhibition patterns were confirmed from linear replots of the slopes or intercepts versus the inhibitor concentrations (not shown). The inhibition constants were calculated from the fits of the complete data set to the corresponding equations from linear competitive inhibition (C) v = Vmax *[S]/(Km(1+[I]/Ki)+[S]), linear noncompetitive inhibition (NC), or linear mixed inhibition (MT) v = Vmax *[S]/Km(1+[I]/Ki) + [S](1+[I]/αKi)), where α = 1 and α Ki is the inhibition constant.