Transcriptomics analysis of 12 melanoma cell lines

Established cancer cell lines often display a relatively homogeneous phenotype and loss of phenotypic heterogeneity compared to the tumours which they are derived from and likely reflect specific phenotypic states within the tumours that are then fixed and maintained under culture condition. Whilst this is often seen as a limitation, it also presents an oportunity to simplify the interpretation of any given experiment and allows us to better understand how distinct phenotypic states within melanoma response/behave in a given experimental setting (Louphrasitthiphol et al., 2019). To this end, we carry out transcriptomics analyses of 12 commonly used melanoma cell lines to better characterize their profiles. Overall design: Indicated cell lines were grown in their respective culture media, 5 biological replicates were collected, of which random three were selected, total RNA extracted and sequenced using 3'-end sequencing