Effect of various inhibitors on propanal oxidation by AtraAOX2.
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AtraAOX2 (1 µg) was pre-incubated with inhibitors for 3 min at 30°C before reaction was started with 2 mM propanal as a substrate. Enzyme activity was determined at 30°C and quenched with 10% acetic acid. The reduction of MTT (at 570 nm) was measured spectrophotometrically. Data are expressed as the mean of thee assays (n = 3).
创建时间:
2015-12-02



