RNA-seq data from ZmHsfA5 transgenic maize plants and wild-type plants under heat stress for article "Heat shock transcription factor ZmHsfA5 coordinates with ZmHsp9 to enhance thermotolerance in maize"

Transcriptome analysis utilized RNA extracted from three-leaf-stage leaves of ZmHsfA5 overexpression (OE) lines and wild-type (WT) plants; samples were collected at 45°C for 0 h and 45°C for 3 h, respectively. Three plants were pooled as one a single biological replicate, with three independent biological replicates prepared per line. cDNA library preparation and high-throughput sequencing were performed by GENEWIZ on an Illumina HiSeq 2500 platform.