Data_2020_Utrecht

We allowed the rhizosphere bacterium Pseudomonas protegens CHA0 to evolve on the roots of Arabidopsis thaliana for six plant growth cycles. To quantify changes in bacterial phenotypes, sixteen evolved bacterial colonies were randomly selected from each of the five plant replicate selection lines at the end of the second, fourth and sixth growth cycle, in addition to sixteen randomly selected ancestral colonies (in total 256 isolates). Then k-means analysis was applied to determining novel bacterial phenotypes. A subset of isolates of each bacterial phenotypes was characterised for their effects on plant root architecture and above and belowground biomass in separate assays at the end of the selection experiment.

本研究允许根际细菌Pseudomonas protegens CHA0在拟南芥Arabidopsis thaliana的根系上经历六个植物生长周期以进行进化。为量化细菌表型的变化，于第二、四和第六个生长周期结束时，从五个植物重复选择系中随机选取了每个进化细菌菌株的十六个菌株，并额外选取了十六个随机选择的祖先菌株（总计256个分离株）。随后，运用k-means分析技术确定了新型细菌表型。在每个细菌表型中选取的分离株子集，在筛选实验结束时，分别进行了对其影响植物根系结构和地上地下生物量的独立检测。