In vitro transcription (IVT) sequencing data for Cap‑STRIPE protocol development

This dataset contains raw sequencing reads and processed data from an in vitro transcription (IVT) experiment designed to evaluate MMLV reverse transcriptase slippage at the 5' cap structure during template-switching. A DNA fragment encoding EGFP under the T7 promoter was amplified by PCR and used as template for capped RNA synthesis with the EasyCap T7 Co‑transcription Kit (Vazyme, DD4203). Template‑switching reverse transcription was then performed using a TSO containing the rGrGrG motif under standard Cap‑STRIPE conditions, and the resulting cDNA was converted into an Illumina‑compatible sequencing library. The library was sequenced on an Illumina NovaSeq X Plus platform to generate 150‑bp paired‑end reads.IVT-1 to IVT-3: three independent IVT replicates; _1 and _2: paired-end read 1 and read 2 files.