Data for "Not dear neighbours in the ant Lasius niger"

This README accompanies the files "Rmarkdown_Cordonnier.pdf", “data_final.txt”, and “Genotypes.xlsx”

Rmarkdown_Cordonnier.pdf

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Rmarkdown for ‘Not dear neighbours: Antennation and jerking, but not aggression, correlate with genetic distance and physical distance in the ant Lasius niger’

Data_final.txt

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Dataset associated with the Rmarkdown

The data are in table form with TABs as variables field delimiters so they can 
be readily imported in any statistical package or spreadsheet program. Please, 
contact me if you need the file formatted otherwise. 

This file includes a description of the variables.

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Variable names and descriptions

date                                          date of the experiment

time                                          time of the experiment

temp_outdoor                        outdoor temperature during the experiment

temp_indoor                          indoor temperature during the experiment

pair_ID                                     ID of the pair of individuals tested in the experiment

target_col                               ID of the target colony tested in the experiment

comp_col                                ID of the competitor colony tested in the experiment

distance                                  geographical distance between the colonies involved in the experiment

group                                       allospecific (interactants from different species),

                                                  nestmate (interactants from the same species, same colony),

                                                  non-nestmate (interactants from same species, different colonies)

neighbour                               neighbour interactants (adjacent slabs) versus non-neighbour interactants

neutral_body_contact          number of neutral body contact between the ants during the interaction

antennation                            number of occurrences of antennation during the interaction

jerking                                      number of occurrences of jerking during the interaction

mandible_gaping                  number of occurrences of mandible gaping during the interaction

brief_biting                             number of occurrences of brief biting during the interaction

gaster_flexing                        number of occurrences of gaster flexing during the interaction

fighting_prolonged_biting   number of occurrences of fighting or prolonged biting over the interaction

RQG_Col                                 relatedness between pairs of colonies (estimator of Queller & Goodnight)

Genotypes.xlsx

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Genotypes of the workers

IDcolony                         ID of the source colony of the genotyped worker

Ant1343 to ant2794     list of the 11 microsatellite markers used in the study

The 15 µl PCR reaction volume consisted of 7.5 µl GoTaq® G2 Hot Start Colorless Master Mix (M7433, Promega), 4.5 µl ddH2O, 1.0 µl unlabelled reverse primer (10µM), 1.0 µl labelled forward primer (10µM; labelled HEX, FAM and TET; final concentration of 0.67µM for each primer) and 1 µl DNA (2-10 ng). The PCR consisted of initial denaturation at 94°C (4 min), 33 cycles at 94°C (denaturation, 30 s), 55°C (annealing, 30 s) and 72°C (elongation, 30 s), and a final step at 72°C (1 min). The PCR products were analysed in an ABI PRISM 310 Genetic Analyser (PE Biosystems) after DNA denaturation at 90°C (1 min). Allele sizes were determined using genescan 3.1 software (PE Biosystems).

********************************* CONTACT **********************************
Please contact me at:

Marion Cordonnier
e-mail: [e-mail]

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