Gene expression of neurotransmitter pathways at 6 dp.

A. Gene expression of neurotransmitter pathways at 6 dpf, Glycinergic pathway gene expression. In the 24 hpf dnVDRa induced group we see a significant decrease in glyt1 expression (p < 0.05) and an increase in glyt2 expression (p < 0.05) and in slc32a1 expression (p < 0.01). In the 48 hpf dnVDRa induced fish there is a significant increase in glyt2 and slc32a1 expression (p < 0.05). In the 72 hpf dnVDRa induced fish there is a significant decrease in glyt2 expression (p < 0.01). Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend. B. Gene expression of neurotransmitter pathways at 6 dpf, Cholinergic pathway gene expression. There are no altered genes in this panel for the 24 hpf dnVDRa induced fish. In the 48 hpf dnVDRa induced fish there is a significant increase in VAChTa expression (p < 0.001). In the 72 hpf dnVDRa induced fish there is a significant decrease in glyt2 expression (p < 0.05). Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend. C. Gene expression of neurotransmitter pathways at 6 dpf, Serotonergic pathway gene expression. In the 24 hpf dnVDRa induced group we see a significant decrease in slc6a4b expression (p < 0.05). In the 48 hpf dnVDRa induced fish there is a significant increase in ddc (p < 0.05), thp2 (p < 0.01), mao (p < 0.05) and slc6a4b (p < 0.01) expression. In the 72 hpf dnVDRa induced fish there is a significant decrease in mao (p < 0.01) and slc6a4b (p < 0.05) expression). Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend. D. Gene expression of neurotransmitter pathways at 6 dpf, GABAergic pathway gene expression. In the 24 hpf dnVDRa induced group we see a significant increase in gad67 expression (p < 0.05). In the 48 hpf dnVDRa induced fish there is a significant increase in gad65 (p < 0.01) and gad67 (p < 0.05) expression. In the 72 hpf dnVDRa induced fish there is a significant decrease in gad67 (p < 0.05) and glsa (p < 0.05) expression. Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend. E. Gene expression of neurotransmitter pathways at 6 dpf, Glutaminergic pathway gene expression. In the 24 hpf dnVDRa induced group we see a significant increase in slc17a6b (p < 0.05) expression). In the 48 hpf dnVDRa induced fish there is a significant increase in slc17a7a (p < 0.01), slc17a6a (p < 0.01) and slc17a6b (p < 0.05) expression. In the 72 hpf dnVDRa induced fish there is a significant decrease in glsa (p < 0.05), glula (p < 0.01), glulb (p < 0.05) and slc17a6a (p < 0.05) expression. Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend. F. Gene expression of neurotransmitter pathways at 6 dpf, Dopaminergic pathway gene expression. In the 24 hpf dnVDRa induced group we see a significant increase in dat and vmat expression (p < 0.05) and a decrease in th1 (p < 0.05), th2 (p < 0.01) and nr4a2a (p < 0.05) expression. In the 48 hpf dnVDRa induced fish there is a significant increase in dbh (p < 0.01), dat (p < 0.05) and manf (p < 0.001) expression. In the 72 hpf dnVDRa induced fish there is a significant decrease in dbh (p < 0.01) and th2 (p < 0.05) expression. Values represent the mean by separate measurements in individual fish with 3–4 fish per gene. Error bars represent the standard deviation of each group. An unpaired t-test with a Welch’s t-test was used for groups when variances were significantly different. Asterisks denote statistical significances as listed above in legend.
(ZIP)