Clustering of up- or downregulated genes to determine biological pathways affected by edelfosine in human CD4+ T cells after gene expression analysis.

(A) The incubation of unstimulated cells with 10 µg/ml edelfosine resulted in the upregulation of apoptosis- and cell death-associated genes. Genes involved in immune response and antigen processing and presentation were downregulated. (B) In the case of stimulated cells which were cultured in presence of 3.3 µg/ml edelfosine the downmodulation of cell cycle progression-related genes was found. Additionally, edelfosine resulted in the upregulation of genes assigned to immune response- and virus response-pathways characterized by type I IFN-regulated genes.