Transcriptome analysis of response to exogenous trehalose and abiotic stress in grapes

Grapevine is an important economic fruit tree, and European grape (Vitis vinifera L.) has been widely used in fresh food, drying, winemaking and grape seed extract. However, most European grapes have low resistance to low temperature, drought and salt stress, and these abiotic stresses will limit the growth and development of grapes, thereby affecting the grape quality and yield. Many reports have shown that exogenous or endogenous trehalose can help improve plant stress resistance. Therefore, in order to investigate the function and molecular mechanism of trehalose metabolism in grape response to stress, this project was conducted. Collect grape leaves from the control group, trehalose, different stresses (low temperature (4 ℃), PEG6000, NaCl), and trehalose+different stress treatments for 48 hours, and perform RNA Seq analysis. The control group and the treatment group each contain three biological replicates. 8 samples examined: CK, 4C, NaCl，PEG6000，Tre，Tre-4C，Tre-NaCl，Tre-PEG6000.