We measured global RNA levels using RNA-seq in Δcas3 E. coli cells with intact CRISPR arrays or cells with the CRISPR-I array deleted to determine if off-target events driven by endogenous spacers aff

NIAID Data Ecosystem30

Homo sapiens Raw sequence reads

CRISPR脱靶效应

基因编辑机制

We modified a long-range amplicon sequencing method with high accuracy to determine CRISPR-induced large deletions by developing a novel k-mer sequence alignment algorithm. We observed that the large

NIAID Data Ecosystem10

Data Sheet 4_Impact of IS26 mobilization on genetic manipulation of multidrug-resistant Acinetobacter baumannii.pdf

细菌基因编辑

抗生素耐药机制

IntroductionMultidrug-resistant Acinetobacter baumannii poses significant challenges for genetic manipulation, historically hindering research on this organism. MethodsTo elucidate the factors contrib

NIAID Data Ecosystem30

Seven CRISPR-Cas9 benchmark off-target datasets used in our study.

CRISPR脱靶效应

基因组编辑安全性评估

Six of them include gRNA-target pairs with mismatches only, and one of them (CIRCLE, denoted with an asterisk) includes gRNA-target pairs with both mismatches and indels. Minority class samples corres

NIAID Data Ecosystem10

Escherichia coli Raw sequence reads. Escherichia coli

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