Transcriptome analysis to identify the genes evoked by nitric oxide

The enzyme DNA methyltransferase (DNMT) catalyzes the methylation of the 5-carbon (C5) position of cytosine in mammals. DNA Methylation regulates gene transcription and serves a variety of physiological and pathophysiological roles. However, the chemical mechanisms regulating DNMT enzyme activity remained largely unknown. Previously, we investigated if nitrosative stress affects the DNA methylation patterns in cells (DRA Accession: DRA012230 ). In this study, we performed RNA-Seq analysis to identify the genes in response to NO donor in HeLa cells. Total RNA was extracted using RNeasy Mini Kit (Qiagen). Library preparation was performed with SureSelect strand-specific RNA library preparation kit (Agilent Technologies), followed by paired-end sequencing on an Illumina NextSeq 550 system (Illumina).