APOE 5'UTR methylation pattern analysis in blood and brain tissue from Alzheimer's disease affected patients

The database includes the raw data of the article “APOE 5’UTR methylation pattern analysis in blood and brain tissue from Alzheimer’s disease affected patients”. The aim of this work was to determine the methylation level of two regions within APOE 5’UTR and to correlate these data with clinical features. The database comprises data obtained by the following investigations: a) Pyrosequencing analysis to evaluate the methylation levels of R1 and R2 CpG sites within APOE 5’UTR; b) Genotype assessment of both patients and healthy controls; c) RT-qPCR for the evaluation of the expression level of APOE in HIC tissue; d) Chemiluminescent enzyme immunoassay to determine the CSF levels of t-TAU, pTAU181, Aβ42 and Aβ42/Aβ40.

A differential methylation was observed in R1 and R2 in both analyzed tissues and interestingly an increase in methylation was observed to be associated with the disease condition in R1. The increase in R1 methylation appeared to be marked when the patients genotype was ɛ4+. In both PBMC and HIC tissues the increase in R1 methylation appeared to be directly proportional to increasing age. Finally, the methylation score obtained by one CpG site in R2 proved to also be related to CSF biomarkers levels.

The outcome highlighted a differential methylation in APOE 5’UTR at least in AD patients PBMCs which seemed to be associated also to APOE genotype, age and CSF biomarkers level.