Integrative Proteome, Transcriptome and DNA Methylome Analysis of Regulatory Networks During U937-derived Macrophage Polarization from an M2 to M1 Phenotype [array]

Tumor associate macrophages (TAMs) depletion or re-education towards an antitumor effector phenotype are considered a promising cancer therapeutic approach. However, the underlying molecular mechanisms remain unclear. Here, we apply proteome, transcriptome and DNA methylome to assay the regulatory networks during polarization of U937-derived macrophages transitioning from an M2 to M1 phenotype. We investigated the differential expression profiles of mRNA and lncRNA in U937 cells between two groups: the M2-U937 (M2 macrophages induced from M0 U937 cells), the M1 U937 (M1 macrophages produced from M2 macrophages). The data of each group are collected from independent experiments in triplicate. Transcriptomic analysis was carried out using a microarray (Arraystar Human lncRNA Gene Expression Microarrays V3.0; Agilent Technology, Santa Clara, USA), which includes about 30,586 LncRNAs and 26,109 coding transcripts.