PRPF8 controls alternative splicing of PIRH2 to modulate the p53 pathway and survival of human embryonic stem cells

To investigate the function of PRPF8 in survial in hESCs, we established hESC cell lines H9 in which PRPF8 has been knocked down by siRNA.We then performed gene expression profiling analysis and alternative splicing analysis using data obtained from RNA-seq of 4 different cells at two time points. Comparative gene expression profiling analysis of RNA-seq data for NT cells and P8i-3 cells. Alternative splicing analysis of RNA-seq data for NT cells and P8i-3 cells.