Leptospira strains used in the present study and results of the real time PCR assays using the species-specific probes and flanking primers.

1INIAV - Instituto Nacional de Investigação Agrária e Veterinária, Lisbon, Portugal. IHMT - Instituto de Higiene e Medicina Tropical, Lisbon, Portugal. KIT - Royal Tropical Institute, Amsterdam, The Netherlands;2Set 1 targets the lipL32 gene of pathogenic Leptospira spp.;3Set 2 targets the secY gene of L. interrogans;4Set 3 targets the ompL1 gene of L. borgpetersenii;5Set 4 targets the secY gene of L. kirschneri;6Set 5 targets the secY gene of L. noguchii; nd - not done; Amplification (+) or no amplification (−).Leptospira strains used in the present study and results of the real time PCR assays using the species-specific probes and flanking primers.