Data underlying the research on Gut microbiota from patients with Long COVID persisting for 2 years result in alterations in mice that resemble post-COVID symptoms

Human gut microbiota and its metabolites were assessed in a healthy control group unexposed to SARS-CoV-2 and an LCOVID group in Hainan, China, using Shotgun metagenomics and liquid chromatography-mass spectrometry of feces. The causal role of the microbiota in LCOVID was further validated by transplanting feces from the subjects into ABx mice using Histopathology and 16S rRNA sequencing.

本研究在中国海南开展，针对未暴露于新型冠状病毒（SARS-CoV-2）的健康对照组与长新冠（LCOVID）组受试者，采用粪便样本的鸟枪宏基因组学（Shotgun metagenomics）与液相色谱-质谱联用法（liquid chromatography-mass spectrometry），对其肠道菌群及其代谢产物开展检测与分析。随后通过将受试者粪便移植至ABx小鼠体内，并结合组织病理学（Histopathology）与16S核糖体RNA测序（16S rRNA sequencing），进一步验证了肠道菌群在长新冠发病过程中的因果作用。