Impact of dietary serine and glycine availability on plasma and tissue metabolite composition in mice

This study examines how altering systemic serine and glycine availability through dietary intervention affects breast cancer metastasis in vivo. Female NOD-SCID-gamma (NSG) mice were maintained on either a control amino acid–replete diet or a serine/glycine-enriched diet for two weeks before and during metastatic progression of human breast cancer cells. By integrating dietary manipulation with in vivo metastasis assays, this study tests whether changes in circulating and tissue nutrient levels can influence metastatic colonization and tumor growth across organs. These data provide insight into how nutrient availability shapes the metabolic environment and metastatic potential of cancer cells.