Gene expression profile at single cell level of hESC H9 derived hemognic niches at Day 18 of in vitro differentiation

Small drug (SB/CHIR) treatment and orbital shear treatment promotes the generation of hemogenic endothelium and multipotent hematopoieitic progenitor cells. We used single cell RNA sequencing (scRNA-seq) to analyze the diversity of hemogenic niches derived from hESC H9 line at day 18 of in vitro differentiation. Overall design: Hemogenic niches derived from day 18 hESC H9 differentiation were harvested and anlyzed using scRNAseq. a 22 balanced experimental design was used to quantify the effect of circuilatory flow (shear) and small drug treatment (SB/CHIR).