Placental ischemia disrupts DNA methylation patterns of distal regulatory regions in rat

We carry out whole-genome bisulfite sequencing (WGBS) of rat placental DNA to investigate epigenome-wide alterations in a reduced uterine perfusion pressure (RUPP) PE model. The Hidden Markov Model (HMM) was applied to identify functional epigenetic structures including partially methylated domains (PMDs), low methylated regions (LMRs) and unmethylated regions (UMRs). Overall design: Whole genome bisulfite sequencing (WGBS) of six placenta samples, including three reduced uterine perfusion pressure (RUPP) rat placenta samples and three control placenta samples