Strains, plasmids, and oligonucleotides used in this work.
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aKnR, Kanamycin resistance; CmR and cat, Chloramphenicol resistance; ErmR and erm, Erythromycin resistance; AmpR, Ampicillin resistance; NxR, Nalidixic acid resistance; SpR, Spectinomycin resistance; CbR, Carbenicillin resistance. Plasmid replication origins are designated oriV with a subscript indicating the source, and oriT indicates the RP4 origin of transfer.bOligonucleotides are in the 5′ to 3′ orientation with introduced restriction sites underlined.
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2015-12-02
