T. b. brucei bloodstream forms survived in A. coluzzii midguts at least 48h post-ingestion.

Mosquito midguts were extracted at day 2 and day 5 post-feeding. Results of three independent experiments are shown in the table, where the numbers (No.) of positive midguts (carrying fluorescent T. b. brucei parasites) over the total number of dissected mosquitoes were counted. The prevalence column shows the proportion (in %) of mosquito midguts carrying fluorescent trypanosomes, at day 2 and day 5 post-trypanosomes feeding. The mean of prevalence is shown in bold for both day 2 and day 5 time points. The lower total numbers of mosquitoes at day 5 are not due to increased mosquito mortality but to the sampling design.