Gene expression profile at single cell level of the placenta from pregnant mice undergoing circadian disruption.

The placenta is composed of distinct cell types. Single cell RNA sequencing (scRNA-seq) was performed to analyze the diversity of cell types contained in the placenta. C57BL/6J pregnant mice were randomly divided and housed under either the control 12h-light/ 12h-dark condition (CON) or chronically phase-shifted condition (CPS) where phase was advanced by 8 hours every 5 days exclusively during pregnancy. On embryonic day 18.5, the placenta was collected and analyzed using scRNA-seq.胎盘由多种不同的细胞类型构成。本研究采用单细胞RNA测序（scRNA-seq）技术，以分析胎盘中的细胞类型多样性。选取C57BL/6J品系孕鼠，随机分为两组，分别饲养于两种光照条件：对照组（CON）为标准12小时光照/12小时黑暗的正常光照周期；慢性相移组（CPS）则仅在妊娠期间每5天将光照周期提前8小时。于胚胎发育第18.5天时，收集胎盘组织并通过scRNA-seq进行测序分析。