RNA-seq analysis after STXBP5 overexpression or STXBP5 knockdown in HA-progerin HEK293 cells

Hutchinson-Gilford Progeria Syndrome (HGPS) is caused by a mutant LMNA called progerin. To determine the mechanism of STXBP5 on progerin, we over expressed STXBP5 or knocked down STXBP5 in HA-progerin HEK293 cells, then analyzed the effect on the expression of coding genes. In this study, we identified STXBP5 as an influencing factor for HA-progerin HEK293 cells. Lowering the expression of STXBP5 may be a new therapeutic strategy for treating age-related phenotypes in HGPS. In order to gain insight into the function of the identified related STXBP5 and progerin, HA-progerin HEK293 cells with over vector-FLAG (Control-OE), over vector-FLAG-STXBP5 (STXBP5), gAAVS1, gSTXBP5 respectively were used to performed four groups of RNA-sequencing (RNA-seq) gene expression profiling of HA-progerin HEK293 cells.