Mauricio Valerio-Santiago, Fernando Monje-Casas (2011) CIL:13888, Saccharomyces cerevisiae. CIL. Dataset

Tem1 (green) normally localizes preferentially to the spindle pole body (SPB) that enters the daughter cell during anaphase; however, Tem1's ability to localize to the SPB is severely decreased in bfa1Δ cells (this image). tem1Δ::GAL-UPL-TEM1 cells expressing eGFP-TEM1 from a CEN plasmid were grown on 2% raffinose/2% galactose and transferred to 2% glucose medium prior to image capture. A differential interference contrast (DIC) image is also shown (hidden). The tem1Δ::GAL1-UPL-TEM1 strain allows for the rapid, conditional depletion of Tem1. UPL, which stands for ubiquitin-proline-LacI, acts as a destabilizing module that permits rapid degradation of appended proteins. Image is Fig 7A, top panels, in J Cell Biol. (2011) 192: 599-614. Images in Fig 7 include CIL #13888, 13872, 13813, 13889, 13874.

Tem1（绿色）通常优先定位于纺锤体极体（SPB），该极体在分裂中期进入子细胞；然而，在bfa1Δ细胞中，Tem1定位于SPB的能力显著降低（如图所示）。tem1Δ::GAL-UPL-TEM1细胞通过CEN质粒表达eGFP-TEM1，并在2%拉法诺糖/2%半乳糖培养基中培养，并在图像捕捉前转移到2%葡萄糖培养基中。此外，还展示了一幅差分干涉对比（DIC）图像（隐藏）。tem1Δ::GAL1-UPL-TEM1菌株允许对Tem1进行快速、条件性的耗竭。UPL（代表泛素-脯氨酸-LacI）作为一种不稳定性模块，能够促进附加蛋白的快速降解。图像为《细胞生物学杂志》（2011）第192期第599-614页中的图7A，顶部面板。图7中的图像包括CIL #13888、13872、13813、13889、13874。