Assessing proliferation frequency and history of Vγ1.1 T cells.

(A) Representative FACS plots of thymic Vγ1.1 γδ T cells from one-week old lckCre;Tlox neonates. Vγ1.1 cells were identified in the gated area of TCRδ and Vγ1.1 doubly stained total thymocytes (left) and used in subsequent display of BrdU incorporation (middle) and GFP/dtTomato expression (right). (B) Analyses of 5-week old mice as described in (A). (C) Analysis of Vγ1.1 T cells present in lymph node (left), spleen (middle), and liver (right) of 5-week old mice as described in (B). Frequency of tdTomato expression was shown in the contour plot below each dot plot. (D) Summary of tdTomato expression frequency in Vγ1.1 T cells obtained from (A–C). Sample sizes are three for one-week old and three for 5-week old mice.