Microarray analysis of E2a-/- and E2a/-Lef1F/F leukemia lines after retroviral Cre expression
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https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE196391
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We compared the transcriptome of E47-/- and E2a/-Lef1F/F leukemia lines 72 hours after transduction with MigR1 or MigR1-Cre. The GFP+ populations were isolated by flow cytometric sorting E2a-/- and E2a/-Lef1F/F leukemia lines were infected with retrovirus producing GFP (MigR1) or Cre (Migr2-Cre) and GFP+ cells were sorted 72 hours after infection. RNA was isolated from sorted cells using Trizol and subject to microarray analysis of 430.a arrays
创建时间:
2022-04-07



