Transcriptomic analysis of Babesia infection in the midgut of ticks
收藏NIAID Data Ecosystem2026-05-10 收录
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https://www.ncbi.nlm.nih.gov/sra/SRP620651
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The enriched RNA was fragmented and reverse-transcribed into cDNA using random hexamers. Second-strand cDNA was synthesized, purified, and used to construct sequencing libraries through end repair, A-tailing, and adapter ligation. Strand-specificity was achieved by incorporating dUTP during second-strand synthesis and subsequent degradation with Uracil-N-Glycosylase (UNG)
创建时间：
2025-09-19
