Transcriptome of Bathylasma hirsutum

Bathylasma hirsutum specimens were collected live in a basin at 978 m near a hydrothermal vent in the mid-Atlantic semi-bathyal region using an ROV. As soon as the barnacle specimens were brought to the deck of the research vessel, they were submerged in RNAlater overnight and then kept at -80. Five B. hirsutum specimens were dissected. Prior to dissection, the sample was thawed at room temperature. Dissection was conducted using a sterilized razor blade. The prosoma was first separated from the wall plates and the operculum plates. For each B. hirsutum specimen, the cirri, maxillopods, testis were dissected. The rim and the mantle tissue underneath the operculum plate, which were densely pigmented with dark purple color and was attached to the edge of the operculum plate, were also isolated.RNAseq libraries were prepared from each total RNA sample using Illumina TruSeq RNA sample Prep Kit v2 and sequenced with the Illumina GAIIx platform at PE150 mode.