Adapted Y-50049 vs. Wild type Y-12632 in response to furfural-HMF challenge

Transcriptional profiling of adapted tolerant industrial yeast Saccharomyces cerevisiae NRRL Y-50049 compared with its parental wild type NRRL Y-12632 in response to challenge of furfural and HMF each at 20 mM. Y-50049 is able to detoxify toxic furan aldehydes in situ while produce ethanol. Under the same conditions, the wild type Y-12632 was repressed and unable to grow and function. Overall design: Two-condition experiment, Y-50049 vs Y-12632 cells. Biological replicates: 2. Independently grown and harvested. Sample time-point at 0, 0.2, 0.5, 1, 2, 18, 24, and 28 hours. Triplicated array with 48 replications of reference control genes. Pre-scan mini array applied.

对适应性耐受型工业酵母Saccharomyces cerevisiae NRRL Y-50049的转录组学分析，与野生型NRRL Y-12632相比，在20 mM的糠醛和HMF挑战下的响应。Y-50049能够在原位解毒有毒的呋喃醛类物质的同时产生乙醇。在相同的条件下，野生型Y-12632受到抑制，无法生长和发挥功能。整体设计：双条件实验，Y-50049与Y-12632细胞对比。生物重复：2次。独立培养和收获。样本时间点为0、0.2、0.5、1、2、18、24和28小时。采用48个重复的参考基因三重阵列。应用预扫描迷你阵列。